What is a Caco-2 cell?

Caco-2 [Caco2] are epithelial cells isolated from colon tissue derived from a 72-year-old, White, male with colorectal adenocarcinoma. This cell line is a suitable transfection host and has applications in cancer and toxicology research.

How do Caco-2 cells grow?

The cells should be kept at 37 °C in a humidified atmosphere containing 5 % CO2. For propagation in culture flasks, Caco-2 cells are seeded in a concentration of 105 cells/cm2. Medium should be changed every 3 days. At 80 % confluence, typically after 4–5 days, the cells are split 1:10 before further cultivation.

What is ATCC cell line?

ATCC or the American Type Culture Collection is a nonprofit organization which collects, stores, and distributes standard reference microorganisms, cell lines and other materials for research and development.

How do you differentiate Caco-2 cells?

Standard methods used to differentiate Caco-2 cells involve seeding them on culture inserts fitted with polycarbonate filters, and allowing spontaneous differentiation to proceed for two to three weeks in culture medium containing 10% or 20% foetal bovine serum (FBS) in both the apical (AP) and basolateral (BL) …

Why are Caco-2 cells used?

The Caco-2 cells isolated from human colorectal adenocarcinoma are widely used to screen for absorption rate of new compounds in the initial stage of drug discovery.

Do Caco-2 cells produce mucus?

However, Caco-2 cells are mostly reported as non/low-mucus producing cells and do not or hardly express mucin-related genes, such as MUC-2 and MUC-625.

How does Caco-2 assay work?

Caco-2 permeability assay to investigate intestinal permeability. Cyprotex’s Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarised Caco-2 cell monolayers and from which the data generated can be used to predict in vivo absorption of drugs.

How do you use ATCC culture?

1 Thaw and aseptically transfer the entire contents of the liquid nitrogen vial to a tube containing 5-6 mL of fresh recommended medium. Mix well. 2 Transfer one-tenth of the cell suspension to two or three other tubes of fresh medium. 3 Incubate the bacteria at 32°C to 37°C under microaerophilic conditions.

What is ATCC type strain?

A type strain may be provided to requestors from a stock initially propagated by the depositor or from the ATCC manufactured and authenticated inventory. Full inclusion as an ATCC General Collection strain will be dependent upon factors such as the need by the scientific community.

What are HT 29 cells?

HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology. These cells are sensitive to the chemotherapeutic drugs 5-fluorouracil and oxaliplatin, which are standard treatment options for colorectal cancer.

What is permeability caco2?

What is Caco-2 assay?

How do you thaw Caco-2 cells?

Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes.

What is caco2 assay?

What is Caco-2 used for?

Caco-2 cells are widely used as an in vitro model for predicting human drug absorption. The Caco-2 cell line is derived from a human colorectal carcinoma, and when cultured, the cells spontaneously differentiate into monolayers of polarized enterocytes.

Why do we use ATCC strains?

Purified viruses. ATCC provides high-titer, purified viruses that support respiratory disease research. Our collection includes influenza virus, human rhinovirus, and human respiratory syncytial virus.

What is HCT116 cell line?

Description. HCT116 (ATCC® CCL-247™) is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised mice, the cells form primary tumors and distant metastases.

What is Caco-2 permeability assay?

Cyprotex’s Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarised Caco-2 cell monolayers and from which the data generated can be used to predict in vivo absorption of drugs. The Caco-2 cell line is derived from a human colon carcinoma.

What is good permeability Caco-2?

Based on in vitro/in vivo correlation studies, Papp values obtained from Caco-2 barrier studies are used to define and predict the intestinal permeability of the tested compounds….Caco-2 Permeability Assay Protocol.

In vitro Papp values	Range of predicted in vivo absorption
Papp ≤ 10-6 cm/s	Low (0-20%)
10-6cm/s < Papp ≤ 10 x 10-6 cm/s	Medium (20-70%)