What is CCCP used for?
What is CCCP used for?
CCCP is a protonophore which is a widely used uncoupler of mitochondrial oxidative phosphorylation. CCCP disrupts ATP synthesis by transporting protons across the mitochondrial inner membrane, interfering with the proton gradient.
How do I make a CCCP solution?
Preparation of 1 mM stock solution (dissolved in Ethanol) for 2-mL O2k-chamber:
- Weigh 1.02 mg of CCCP into a glass vial.
- Dissolve in 5 mL ethanol.
- Divide into 0.2 mL portions in glass vials and store at -20 °C.
Is CCCP toxic?
Causes skin irritation. Material may be irritating to the mucous membranes and upper respiratory tract. May cause respiratory system irritation. Toxic if inhaled, swallowed, or in contact with skin.
What is CCCP in mitochondria?
CCCP/FCCP is a lipid-soluble weak acid and a potent mitochondrial uncoupling agent that increases the proton permeability across the mitochondrial inner membranes, thus dissipating the transmembrane potential and depolarizing the mitochondria.
What is CCCP microbiology?
Carbonyl Cyanide m-Chlorophenylhydrazine (CCCP) Reverses Resistance to Colistin, but Not to Carbapenems and Tigecycline in Multidrug-Resistant Enterobacteriaceae – PMC.
What does carbonyl cyanide m Chlorophenylhydrazone do?
CCCP/FCCP is a lipid-soluble weak acid and a potent mitochondrial uncoupling agent that increases the proton permeability across the mitochondrial inner membrane, thus dissipating the transmembrane potential and depolarizing the mitochondria.
How does CCCP affect pH?
The uncoupler, carbonylcyanide-m-chlorophenylhydrazone (CCCP), caused a marked decrease in intracellular pH, ATP and potassium of both strains. Low concentrations of CCCP stimulated glucose consumption rate, but higher concentrations were inhibitory.
What is CCCP in biology?
CCCP (Carbonyl cyanide m-chlorophenyl hydrazone, Carbonyl cyanide 3-chlorophenylhydrazone), an oxidative phosphorylation inhibitor, is a protonophore mitochondrial uncoupler that increases membrane permeability to protons, leading to a disruption in the mitochondrial membrane potential.
What is carbonyl cyanide 3 Chlorophenylhydrazone CCCP?
Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) induces mitophagy in mammalian cells. CCCP suppresses the expression of few electron transport chain (ETC) proteins. Protonophore (H+ ionophore) and uncoupler of oxidative phosphorylation in mitochondria. Shown to have a number of effects on cellular calcium.
