What is Jurkat T cells?

Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.

Who did Jurkat cells come from?

The Jurkat cell line was established from the peripheral blood of a 14 year old boy by Schneider et al., and was originally designated JM. The line was cloned from cells obtained from Dr. Kendall Smith and are mycoplasma free.

Is Jurkat a CD4?

Jurkat is a CD4 T cell line provided by O. Acuto (Institut Pasteur, Paris, France). Kit 225 is a CD4 T cell line expressing high- and intermediate-affinity IL-2R (13). YT is a cell line expressing intermediate-affinity IL-2R.

Is Jurkat CD4 or CD8?

CD4+
Jurkat is a CD4+/CD8- T-cell lymphoma that expresses an endogenous TCR. The T2 line is a TAP-deficient T-cell/B-cell hybridoma expressing HLA-A2.

Why are they called Jurkat cells?

Jurkat cell line was established from the peripheral blood of a 14-year-old boy with acute lymphoblastic leukemia (ALL) at first relapse in 1976; often this cell line is called “JM” (JURKAT and JM are derived from the same patient and are sister clones).

How do you activate Jurkat cells?

Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss. IL-2 production should be measured 6 to 12 h later (whatever works best for you). 0.5 to 2 millions cells per ml works.

What is the HLA type of Jurkat cells?

The Jurkat HLA panel is comprised of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in a Jurkat T lymphocyte parental cell line background. The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines.

Where is HEK293 from?

HEK293 are immortalized human embryonic kidney cells. Originally isolated in the 1970s by Alex Van der Eb, a Dutch biologist, it was postdoc Frank Graham who transformed the cell line with sheared adenovirus 5 (Ad5). The ‘293’ refers to the fact that it was Graham’s 293rd experiment.

Are Jurkat cells CD4 positive?

Jurkat is a human CD4+-T-cell line that expresses CD3 along with a number of other T-cell surface receptors which are typical of freshly isolated CD4+ human peripheral blood T cells.

How do Jurkat cells grow?

1. Maintain Jurkat cells in RPMI with l-glutamine supplemented with 10% fetal bovine serum at 37°C, 5% CO2 in a humidified incubator. Grow cells to no more than 2 × 105 cells/mL and passage cells 2–3 times a week.

Are Jurkat cells activated?

Jurkat T cells were activated by anti-CD3, anti-CD28, PMA, or ionomycin or combinations of these single stimuli, in order to map the contribution of these stimuli towards the activation of proximal, medial and distal signal transduction pathways.

Do Jurkat cells express HLA?

Features and Benefits. Unlike wild type Jurkat cells, which express multiple HLA subtypes, the Jurkat HLA panel cell lines express individual MHC Class I HLA subtypes. These monoallelic HLA cell lines can be used to study peptide binding and presentation in the context of individual HLA subtypes.

Is HEK293 cancerous?

Thus, HEK293 is not a model for normal human cells; these cells are immortalized already by known oncogene but not malignant yet.

What have HEK293 cells been used for?

HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.

How do you maintain a Jurkat cell?

1) Immediately place frozen cells in liquid nitrogen storage incubator. 2) Quickly thaw ampoule in 37oC water bath. 3) Transfer thawed cells to a T25 flask with 10ml of warm growth media. 4) Allow cells to recover over night in 37oC, 5% CO2 humidified incubator.

Why do T cells express MHC II?

Abstract. The main function of major histocompatibility complex (MHC) class II molecules is to present processed antigens, which are derived primarily from exogenous sources, to CD4(+) T-lymphocytes. MHC class II molecules thereby are critical for the initiation of the antigen-specific immune response.

Where did HEK293 come from?

HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA.

What is the difference between MHC I and MHC II?

The main difference between MHC class 1 and 2 is that MHC class 1 molecules present antigens to cytotoxic T cells with CD8+ receptors whereas MHC class 2 molecules present antigens to helper T cells with CD4+ receptors.

What is the role of MHC II?

What are the functions of MHC I and MHC II?

MHC and antigen presentation MHC class I molecules present antigens that are intracellular or endogenous, whilst MHC class II molecules present antigens that are extracellular or exogenous.