What is a typical fluorescence lifetime?

The average time the electron stays in an excited state before photon emission is referred to as the lifetime. Fluorescence lifetime typically ranges in the order of 10− 9 to 10− 7 [s].

Does nile red fluorescence?

Nile red exhibits properties of a near-ideal lysochrome. It is strongly fluorescent, but only in the presence of a hydrophobic environment. The dye is very soluble in the lipids it is intended to show, and it does not interact with any tissue constituent except by solution.

How does fluorescence lifetime imaging work?

Fluorescence Lifetime Imaging (FLIM) produces an image based on the differences in the excited state decay rate from a fluorescent sample. Thus, FLIM is a fluorescence imaging technique where the contrast is based on the lifetime of individual fluorophores rather than their emission spectra.

Does Nile Red dissolve in water?

In water, the Nile Red derivative 1 showed hardly any solubility at all. Surprisingly, despite having a phenolic-OH, this compound was still insoluble in pH 9 borate buffer (1 M) or in sodium carbonate solution.

What is the difference between radiative lifetime and fluorescence lifetime?

The fluorescence lifetime of a molecule is governed by the competition between radiative and (all) non radiative decay. The longest fluorescence lifetime will be the natural radiative decay rate when all non radiative decay channels are prevented or orders of magnitude longer than radiative decay.

Is Nile Red a real chemist?

After finishing his degree, he worked as a lab tech in an organic lab and took post-graduate studies in applied chemistry. He however ended up leaving his post-graduate studies early to focus on his YouTube channel.

Is Nile Red white?

I am very white washed. I know some basic japanese that I could use as tourist.

What factors affect fluorescence lifetime?

Every substance that is excited and emits photons (thus fluorescence) has its own characteristic fluorescence lifetime. The emission of the autofluorescence material mixes with the emission of the fluorophore of interest. If it is not filtered out, it will influence the measured lifetime.

What is fluorescence imaging used for?

Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy.

Is NileRed white?

What dissolves Nile red?

Nile red is relatively insoluble in aqueous solutions and is first dissolved in acetone, then mixed rapidly with water immediately prior to application to the gel.

What are the advantages of using fluorescence lifetime measurements instead of intensities for Imaging?

2.3 Fluorescent Lifetime Imaging Microscopy An important advantage of FLIM measurements is that they are independent of change in probe concentration, photobleaching, and other factors that limit intensity-based steady-state measurements.

How do you calculate radiative lifetime?

How to calculate radiative and non radiative rates for multiple lifetimes? Typical, radiative rate kr = PLQY/lifetime. Here, lifetime is measured using tcspc ie variation of PL intensity (film or solution) versus time (say nanosec). In case of monoexponential decay, kr can be estimated if PLQY and lifetime is known.

Did YouTube stop NileRed?

NileRed still makes videos, just building yet another new lab, so is going slow, he still uploads shorts on his NileRed shorts channel. Chemplayer has been kicked off YouTube and have a BitChute channel.

Who is behind NileRed?

Against popular theories posed by NileRed’s subscribers, ‘Nile Red’ is not actually the name of the popular channel’s creator. Nigel Braun (pron. ‘brawn’) is the man behind the myth. The young Canadian chemist has been making videos since his early teens, but uploaded his very first YouTube video in March of 2014.

What race is NileRed?

He is of German and Japanese descent.

How can I increase my lifetime fluorescence?

For increasing the fluorescence lifetime of a molecule you have to decrease the Internal Conversion (IC) and Inter System Crossing (ISC). Structural modification is the only way for this.

What are advantages of fluorescence microscopy?

Fluorescence microscopy is one of the most widely used tools in biological research. This is due to its high sensitivity, specificity (ability to specifically label molecules and structures of interest), and simplicity (compared to other microscopic techniques), and it can be applied to living cells and organisms.

Can MRI detect fluorescence?

MRI has become a critical technology for chemists continue to devise new types of MRI contrast agents. The MRI/fluorescence dual-modal detection of GSH provides a solution able to overcome some issues of poor sensitivity and penetration.

How painful is white phosphorus?

White phosphorus causes severely painful, partial (second degree) to full thickness (third degree) burns, which have a characteristic yellow color and garlic-like odor. Smoke may release from the burn site from the continued burning of white phosphorus or the formation of phosphoric acid.

How does polarity affect the fluorescence of Nile red?

Nile Red fluorescence depends on the polarity of the environment in which it is dissolved. In a polar solvent such as water, Nile Red’s fluorescence intensity is very weak and red shifted relative to its properties in a hydrophobic environment, such as a lipid compartment (Greenspan and Fowler, 1985 ).

What can we learn from the Nile red experiment?

As such, Nile Red has the potential to reveal changes that may be opaque to other methods (Greenspan and Fowler, 1985). BODIPY-labeled fatty acids, which are also used as vital dyes to study C. elegans fat metabolism, have a number of properties that distinguish them from Nile Red.

What is the emission maxima of Nile red in the intestine?

The emission maxima in these intestinal compartments reside in the 620–635 nm range. Most emission filters used to capture red emissions (i.e., Cy3, mCherry) efficiently capture Nile Red emissions from this lipid storage site. Because Nile Red’s fluorescence properties are very sensitive to its environment,…

What is the significance of Nile red staining in microalgae?

The Nile red fluorescence staining of oil droplets in microalgae yields brilliant yellow fluorescence, which allows us to examine the size, shape, and number of the oil droplets with confocal or epifluorescence microscopy.