Can I use clear bottom plates for fluorescence?

Fluorescence (FI) bottom: Transparent plates should not be used with fluoresence as reflections or unspecific signals can significantly impair sensitivity.

What is UV Plate?

UV Plate 96 Well, Flat Bottom, Cs 50 …and 280 nm Flat bottom wells have volume of 370 µL UV-Plate has a special material molded onto the bottom that is transparent to UV light. This important feature enables researchers to determine nucleic acid and/or protein concentrations in a 96 well format.

Is polystyrene UV transparent?

Standard polystyrene used in many microplates absorbs light in the UV range, resulting in high background. To minimize background in the UV range, quartz, COC, or microplates made with ‘UV-transparent’ plastics such as fluorocarbon (FC) are often used.

How do you sterilize a microtiter plate?

Sterilization Methods for Microplates

1. Gamma irradiation – typical 15-40kGy (ANSI/AAMI/ISO 11137-1994)
2. E-beam irradiation – typical 15-40kGy (ANSI/AAMI/ISO 11137-1994)
3. High pressure steam (autoclave) – 121°C (250°F), 20min, 15psi (1.03 bar); longer time frames permit lower temperatures and pressures (ISO 17665-1:2006)

What plate is used for fluorescence?

For luminescent assays, white plates reflect light and will maximize light output signal. For fluorescent assays, black plates absorb light and reduce background and crosstalk.

Why do we use black plates for fluorescence?

The primary difference between white and black plates is their reflective properties. For luminescent assays, white plates reflect light and will maximize light output signal. For fluorescent assays, black plates absorb light and reduce background and crosstalk.

Can microplates be reused?

According to the company, the new plates pay for themselves after approximately 5-8 uses, and can be reused many times more.

Can you reuse QPCR plates?

Yes, is possible.