What are the domains of p53?

The p53 family proteins share significant similarity at the amino-acid level within three domains: the transcriptional activation domain (AD), the sequence-specific DNA-binding domain (DBD), and the tetramerization domain (TD) (Figure 1a).

Are p53 and TP53 the same?

The TP53 gene provides instructions for making a protein called tumor protein p53 (or p53). This protein acts as a tumor suppressor, which means that it regulates cell division by keeping cells from growing and dividing (proliferating) too fast or in an uncontrolled way.

What does TP53 stand for?

A TP53 genetic test looks for a change, known as a mutation, in a gene called TP53 (tumor protein 53). Genes are the basic units of heredity passed down from your mother and father. TP53 is a gene that helps stop the growth of tumors.

Does p53 have a DNA-binding domain?

Abstract. The p53 tumor suppressor is a sequence-specific DNA binding protein that activates gene transcription to regulate cell survival and proliferation.

Which domain of p53 is bound by MDM2?

transactivation domain
The MDM2 protein, in turn, can bind the transactivation domain of p53, inhibiting further p53 activity as a transcription factor (6, 11, 12).

What is Tetramerization domain?

Structure of the tetramerization domain A monomer contains a β-strand (residues 326 to 333) linked to an α-helix (residues 335 to 355) by a single residue (glycine-334) (Figure 1a). A monomer has a V-shape, and both elements of the secondary structure are a branch of the V.

What type of mutation is TP53?

Mutations in the TP53 gene are the most commonly acquired mutations in cancer. The p53 protein, made by the TP53 gene, normally acts as the supervisor in the cell as the body tries to repair damaged DNA. Different mutations can determine how well or how poorly that supervisor is able to direct the response.

Why is TP53 called the guardian of the genome?

Dubbed the “guardian of the genome,” the p53 protein (blue) senses damage in DNA (pink) and triggers a protective response. The most commonly mutated gene in cancer, p53, works by shifting cell metabolism in a way that alters a cell’s identity, researchers at the Sloan Kettering Institute have discovered.

What is a protein binding domain?

In molecular biology, binding domain is a protein domain which binds to a specific atom or molecule, such as calcium or DNA. A protein domain is a part of a protein sequence and a tertiary structure that can change or evolve, function, and live by itself independent of the rest of the protein chain.

Is p53 a tetramer?

Abstract. The p53 tumor suppressor protein is a tetramer that binds sequence-specifically to a DNA consensus sequence consisting of two consecutive half-sites, with each half-site being formed by two head-to-head quarter-sites (–><– –><–).