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What is the substage light on a microscope?

What is the substage light on a microscope?

Substage light: Located in the base, the light passes directly upward through the microscope. Stage: The platform the slide rests on while being viewed. The stage has a hole in it to permit light to pass through both it and the specimen. The mechanical stage permits precise movement of the specimen.

What is a substage of a microscope?

Definition of substage : an attachment to a microscope by means of which accessories (such as mirrors, diaphragms, or condensers) are held in place beneath the stage of the instrument.

How do you use an illuminator on a microscope?

Steps on How to Use a Light Microscope

  1. Step 1: Connect the light microscope to a power source.
  2. Step 2: Turn the revolving nosepiece so the lowest objective lens is in position.
  3. Step 3: Mount your specimen onto the stage.
  4. Step 4: Use the metal clips to keep your slide in place.

What is the purpose of Köhler illumination?

In practice, Köhler illumination is used in most microscopes, and a specialized form of critical illumination is used in confocal microscopes. Köhler illumination provides a uniformly illuminated, bright field of view, which is important when using an uneven light source, like a coiled tungsten filament.

What is the substage illuminator?

The substage condenser gathers light from the microscope light source and concentrates it into a cone of light that illuminates the specimen with uniform intensity over the entire viewfield.

Where is the substage on a compound microscope?

The definition of a substage is a device that supports mirrors and other attachments under the common stage of a compound microscope. An example of a substage is what holds a mirror in order to see what is on the slide of a microscope. The stage, below the main stage of a microscope, to which attachments are fixed.

What is the illuminator part of the microscope?

Illuminator is the light source for a microscope, typically located in the base of the microscope. Most light microscopes use low voltage, halogen bulbs with continuous variable lighting control located within the base. Condenser is used to collect and focus the light from the illuminator on to the specimen.

What is a substage condenser?

What is the major difference between critical illumination and Köhler illumination?

The primary limitation of critical illumination is the formation of an image of the light source in the specimen image plane. Köhler illumination addresses this by ensuring the image of the light source is perfectly defocused in the sample plane and its conjugate image planes.

What is substage diaphragm?

The aperture diaphragm is probably the single most important adjustment on the microscope, in that it controls the numerical aperture of the substage condenser, which, in turn, controls the numerical aperture of the objectives, and, therefore, controls the resolving power of the entire system.

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