What is a cytokine assay?

Cytokine assays are an assessment of the preparation and treatment of blood and tissue samples related to cytokines. Cytokine assays are essential in disease diagnosing and monitoring because cytokines are pivotal players in the progression or regression of a pathological process and are biomarkers for many disease.

What is a whole blood assay?

A whole blood assay as a simple, broad assessment of cytokines and chemokines to evaluate human immune responses to Mycobacterium tuberculosis antigens.

How are blood cytokines measured?

Immunoassays: Immunoassays currently are the method of choice for determination of cytokines. Enzyme-linked immunosorbent assay (ELISA) is the commonly used form of immunoassay. ELISA uses a primary antibody for the capture and a secondary antibody conjugated to an enzyme or radioisotope for the detection.

What is whole blood stimulation?

Whole blood stimulation has been used to investigate the cellular responsiveness to a variety of stimuli, including bacterial endotoxin (LPS), antigens, allergens, and antibiotics.

Why do we measure cytokines?

Measurement of circulating cytokine levels can provide important information in the study of the pathogenesis of disease.

What is the meaning of assay in chemistry?

An assay is a process of analyzing a substance to determine its composition or quality. Three techniques – fire assay, wet chemistry, and instrumental analysis – are primarily used to analyze and determine purity of metals.

What is a cytokine panel blood test?

This assay is used for understanding the pathophysiology of immune, infectious, inflammatory disorders and for research purposes. Lab Testing Sections: Flow/Immunology – Sendouts.

Can a blood test measure cytokines?

[Differential diagnosis using blood cytokine levels] Blood cytokine levels are useful and important in the differential diagnosis of inflammatory disorders such as neutrophilia, eosinophilia, and especially in distinguishing tumoral fever from infectious fever in malignant lymphomas.

Are cytokines inflammatory markers?

Cytokines, including interleukins, interferons, tumor necrosis factors, and chemokines, have a variety of pro- and anti-inflammatory effects in the body through a number of biochemical pathways and interactions.

What are the different clinical assays for cytokines?

Immunoassays such as ELISA are currently the most commonly used techniques to quantify cytokines due to the high specificity and sensitivity [10]. Built on the same principle, more rapid, automated, and high throughput methods have been developed [11].