What does EMSA measure?

An electrophoretic mobility shift assay (EMSA, also known as a gel shift assay) is used to determine if a protein is able to directly interact with a short, specific sequence of DNA.

What does the Dnase footprinting assay tell you and what does the EMSA tell you?

DNA Footprinting: This is another DNA-binding assay but it gives precise information about where a protein binds DNA. Normally EMSA is used to narrow down the region followed by DNA footprinting. The target DNA is end-labelled and is then incubated with a purified protein or protein extract.

What is poly dI dC?

Poly(deoxyinosinic-deoxycytidylic) acid (Poly(dI-dC) • Poly(dI-dC)) is an alternating copolymer used as a DNA substrate for evaluation of DNA methytransfeases, such as DNA-methyltransferase 1 and as double-stranded DNA model for conformational studies of DNA structure dynamics and drug, small molecule, interactions.

What does EMSA mean?

EMSA (Emergency Medical Services Authority) is Oklahoma’s largest provider of pre-hospital emergency medical care.

How is EMSA done?

The EMSA technique is based on the observation that protein–DNA complexes migrate more slowly than free linear DNA fragments when subjected to non-denaturing polyacrylamide or agarose gel electrophoresis.

Why is ChIP preferred over EMSA?

In contrast, the chromatin immunoprecipitation (ChIP) offers a distinct advantage over EMSA and in vivo footprinting, since the ChIP technique not only specifies which nucleotides are bound, but also identifies the interacting protein(s) in the context of in vivo samples [7].

What is a probe in EMSA?

In an EMSA, non-denaturing polyacrylamide gel electrophoresis resolves protein–DNA complexes. A radiolabeled DNA probe contains the DNA lesion or DNA structure of interest. The protein–DNA complexes assemble by incubation of the DNA probe with either crude protein extract or purified proteins (Fig. 1).